布鲁氏菌Omp10蛋白抗原表位的生物信息学分析Bioinformatics analysis of Brucella's Omp 10 antigen epitope
杨妍;朱玥洁;胡金伟;张峰波;甫拉提·热西提;张春桃;贾斌;庞盼;丁剑冰;
摘要(Abstract)：
目的用生物信息学技术预测布鲁氏菌Omp10蛋白的抗原表位,寻找布鲁氏菌Omp10的优势表位。方法利用IEDB在线软件、DNAStar软件、Pro Pred MHC Class-ⅡBinding Peptide Prediction Server软件和SYFPEITHI软件,通过分子结构、亲水性、柔韧性、表面可及性以及抗原指数等在线分析Omp10蛋白的二级结构、B细胞抗原表位及T细胞抗原表位。结果布鲁氏菌Omp10蛋白B细胞抗原表位有4个,为26-34、65-75、97-102和115-120氨基酸残基。Omp10蛋白T细胞抗原表位有6个,为64-79、72-87、104-119、6-21、9-24和78-93氨基酸残基。结论通过综合分析布鲁氏菌Omp10蛋白有4个B细胞抗原表位和6个T细胞抗原表位,为进一步筛选具有一定抗原保护性和免疫原性的优势表位和多价疫苗构建奠定了基础。
关键词(KeyWords)： 布鲁氏菌;Omp10蛋白;抗原表位
基金项目(Foundation): 国家自然科学基金(81560322);; 新疆维吾尔自治区自然科学基金(2015211C100);; 新疆医科大学科学创新基金(XYDCX201507)
作者(Author): 杨妍;朱玥洁;胡金伟;张峰波;甫拉提·热西提;张春桃;贾斌;庞盼;丁剑冰;
Email:
DOI:
参考文献(References)：
- [1]Franco MP,Mulder M,Gilman RH,et al.Human brucellosis[J].Lancet Infect Dis,2007,7(12):775-786.
- [2]Arimi SM,Koroti E,Kang'ethe EK,et al.Risk of infection with Brucella abortus and Escherichia coli O157:H7 associated with marketing of unpasteurized milk in Kenya[J].Acta Trop,2005,96(1):1-8.
- [3]Whatmore AM.Current understanding of the genetic diversity of Brucella,an expanding genus of zoonotic pathogens[J].Infect Genet Evol,2009,9(6):1168-1184.
- [4]Forland F,De Carvalho Gomes H,Nokleby H,et al.Applicability of evidence-based practice in public health:risk assessment on Q fever under an ongoing outbreak[J].Euro Surveill,2012,17(3):20060.
- [5]Dean AS,Bonfoh B,Kulo AE,et al.Epidemiology of brucellosis and q Fever in linked human and animal populations in northern togo[J].PLo S One,2013,8(8):e71501.
- [6]Purwar S,Metgud SC,Gokale SK.Exceptionally high titres in atypical presentation of occult epididymo-orchitis due to brucellosis[J].J Med Microbiol,2012,61(Pt 3):443-445.
- [7]Erdem H,Elaldi N,Ak O,et al.Genitourinary brucellosis:results of a multicentric study[J].Clin Microbiol Infect,2014,20(11):847-853.
- [8]Hasanoglu I,Guven T,Maras Y,et al.Brucellosis as an aetiology of septic arthritis[J].Trop Doct,2014,44(1):48-49.
- [9]Baldi PC,Giambartolomei GH.Pathogenesis and pathobiology of zoonotic brucellosis in humans[J].Rev Off Int Epizoot,2013,32(1):117-125.
- [10]Herrick JA,Lederman RJ,Sullivan B,et al.Brucella arteritis:clinical manifestations,treatment,and prognosis[J].Lancet Infect Dis,2014,14(6):520-526.
- [11]Akhvlediani T,Clark DV,Chubabria G,et al.The changing pattern of human brucellosis:clinical manifestations,epidemiology,and treatment outcomes over three decades in Georgia[J].BMC Infect Dis,2010,10:346.
- [12]Ducrotoy MJ,Bertu WJ,Ocholi RA,et al.Brucellosis as an emerging threat in developing economies:lessons from Nigeria[J].PLo S Negl Trop Dis,2014,8(7):e3008.
- [13]董炳梅,王金良,唐娜,等.布鲁氏菌的致病机制与细胞免疫机制研究进展[J].中国人兽共患病学报,2012,28(6):635-639,643.
- [14]马秀敏,胡晓安,阿尔孜古丽·吐尔逊,等.细粒棘球绦虫Ag B1抗原表位的生物信息学预测[J].科技导报,2013,31(27):27-30.
- [15]李江英,白雪娟,梁艳,等.用DNAStar软件预测Rv1410c结核分枝杆菌蛋白抗原表位[J].细胞与分子免疫学杂志,2015,31(4):474-477.
- [16]Liu XL,Zhao H,Cao WY,et al.Bioinformatic prediction of the antigenic epitopes of recombinant ferritin of Echinococcus granulosus[J].Mol Med Rep,2016,13(1):888-894.
- [17]Li YH,Liu XF,Zhu YJ,et al.Bioinformatic prediction of epitopes in the Emy162 antigen of Echinococcus multilocularis[J].Exp Ther Med,2013,6(2):335-340.
- [18]Zhang FB,Lu XB,Guo N,et al.The prediction of T-and Bcombined epitope of Ag85 B antigen of Mycobacterium tuberculosis[J].Int J Clin Exp Med,2016,9(2):1408-1421.