马虎;阿迪力·阿不都热合曼;秦浩;努尔乎玛尔·库尔班;邬利娅·伊明;艾尼瓦尔·吾买尔;

• 1:新疆医科大学基础医学院药理教研室
• 2:新疆医科大学研究生学院

摘要(Abstract)：

目的探讨雌激素对肾血管性高血压大鼠心脏功能及氧化应激的影响。方法取10周龄SD大鼠,狭窄一侧肾动脉建立肾血管性高血压(2KIC)模型,雌性大鼠去除双侧卵巢建立雌激素低下模型(VOX),分为2K1C雌性组(2KIC-F组)、2K1C雌性去势组(2K1C-VOX组)和2K1C雄性组(2K1C-M组)。于手术后16w进行超声心动图检查和心脏血流动力学检查,评价雌激素对肾血管性高血压大鼠心脏结构和功能的影响;取心脏组织液氮冻存,测定心肌组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、总抗氧化能力(T-AOC)及抗超氧阴离子(抗O-2)。结果超声心动图结果显示,与2KIC-F组比较,2KIC-VOX组左心室收缩末期内径(LVIDd)、左心室舒张末期后壁厚度(LVPWd)显著降低,2KIC-M组LVPWd显著升高(P<0.05)。血流动力学结果显示,与2KIC-F组比较,2KIC-VOX组和2KIC-M组收缩压(SBP)、舒张压(DBP)均有明显降低,2KIC-VOX组和2KIC-M组差异无统计学意义;2KIC-M组室内压最大上升速率(+dp/dtmax)、室内压最大下降速率(-dp/dtmin)均显著降低;与2KIC-VOX组比较,2KIC-M组-dp/dtmin显著降低(P<0.05)。氧化应激结果显示,与2KIC-F组比较,2KICVOX组心脏组织SOD的活性升高,MDA含量降低;2KIC-M组SOD的活性、T-AOC及抑制阴离子水平升高,MDA含量降低;与2KIC-VOX组比较,2KIC-M组T-AOC、抗O~-_2、MDA水平显著增高(P<0.05)。结论雌激素可加重肾血管性高血压的严重程度和氧化应激水平,雌激素低下时,其严重程度得以改善。

关键词(KeyWords)： 雌激素;肾血管性高血压大鼠;血流动力学;氧化应激

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(81360048);; 新疆医科大学创新基金(XJC201111)

作者(Author): 马虎;阿迪力·阿不都热合曼;秦浩;努尔乎玛尔·库尔班;邬利娅·伊明;艾尼瓦尔·吾买尔;

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DOI:

参考文献(References)：

• [1]Ohnston CL.Renin-angiotensen system:a dual tissue and hormonal system for cardiovacular control[J].J Hypertens Suppl,1992,10(7):13-26.
• [2]Dzau VJ,Bernstein K,Celermajer D,et al.The relevance of tissue angiotensin-converting enzyme:Manifestations in mechanistic and endpoint data[J].Am J Cardiol,2001,88(9):1-20.
• [3]Bhatia K,Elmarakby AA,El-Remessy AB,et al.Oxidative stress contributes tosex differences in angiotensin II mediated hypertension in spontaneously hypertensive rats[J].Am J Physiol Regul Integr Comp Physiol,2012,302(2):274-282.
• [4]Wen HR,Liu JG,LH Xie.Oxidative stress-mediated effects of angiotensin II in the cardiovascular system[J].World J Hypertens,2012,2(4):34-44.
• [5]Karl D,Pender G,Nancy T,et al.Sex differences in circulating and renal angiotensins of hypertensive mRen(2).Lewis but not normotensive Lewis rats[J].Am J Physiol Heart Circ Physiol,2008,295(1):10-20.
• [6]Oestreicher EM,Guo C,Seely EW,et al.Estradiol increases proteinuria and angiotensin II type 1receptor in kidneys of rats receiving L-NAME and angiotensin II[J].Kidney Int,2006,70(10):1759-1768.
• [7]Seely EW,Brosnihan KB,Jeunemaitre X,et al.Effects of conjugated oestrogen and droloxifene on the renin angiotensin system,blood pressure and renal blood flow in postmenopausal women[J].Clin Endocrinol(Oxf),2004,60(3):315-321.
• [8]Stier CT,Chander PN,Rosenfeld L,et al.Estrogen promotes microvascular pathology in female stroke-prone spontaneously hypertensive rats[J].Am J Physiol Endocrinol Metab,2003,285(1):232-239.
• [9]Christine MB,Michaele B.Sex differences in hypertension:contribution of the Renin–Angiotensin System[J].Gend Med,2012,9(9):291.
• [10]Chappell MC,Westwood BM,Yamaleyeva LM.Differential effects of sex steroids in young and aged female mRen2.Lewis rats:a model of estrogen and salt-sensitive hypertension[J].Gend Med,2008,5(Suppl A):65-75.
• [11]Yanes LL,Sartori-Valinotti JC,Iliescu R,et al.Testosterone dependent hypertension and upregulation of intrarenal angiotensinogen in Dahl salt-sensitive rats[J].Am J Physiol Renal Physiol,2009,296(4):771-779.
• [12]Gallagher PE,Li P,Lenhart JR,et al.Estrogen regulation of angiotensin converting enzyme mRNA[J].Hypertension,1999,33(1Pt 2):323-328.
• [13]Manuel MM.Pathophysiology of renovaseular hypertension[J].Hypertension,1991,17(17):707-719.
• [14]殷晓峰,胡刚,汪海.血压调控机制及抗高血压药物靶标[J].中国药理学通报,2003,19(9):971-975.
• [15]胡剑平,谈世进,丁在咸.雄激素在高盐引发高血压中的作用[J].中华高血压杂志,2008,16(11):991-993.
• [16]Bhatia K,Elmarakby AA,El-Remessey A,et al.Oxidative stress contributes to sexdifferences in angiotensin II-mediated hypertension in spontaneously hypertensive rats[J].Am J Physiol Regul Integr Comp Physiol,2012,302(2):274-282.
• [17]邢之华,蔡昌龙,李明月,等.高血压病患者血压与血清SOD、MDA、CAT、GSH-PX水平的关系[J].江苏医药,2005,31(5):367-368.
• [18]Redon J,Oliva MR,Tormos C,et al.Antioxidant activities and oxidative stress byproducts in human hypertension[J].Hypertension,2003,41(5):1096-1101.