MMP-9和PTEN在乳腺癌中的表达及其临床意义Expression and clinical significance of MMP-9 and PTEN in the tissues of breast cancer
师晓莉;刘存;
摘要(Abstract)：
目的:探讨基质金属蛋白酶-9(MMP-9)和PTEN在乳腺癌中的表达及其临床意义。方法:运用免疫组化Envision方法检测56例乳腺癌和22例乳腺良性病变中(乳腺腺病和乳腺腺瘤各11例)MMP-9和PTEN的表达情况,并分析其与临床病理参数的关系。结果:(1)MMP-9在乳腺癌中的阳性率为69.64%(39/56),在乳腺良性病变中的阳性率为45.45%(10/22);PTEN在乳腺癌中高表达率为46.43%(26/56),在乳腺良性病变中为86.36%(19/22),两组差异有统计学意义(P<0.05)。(2)MMP-9的表达与组织学分级和腋窝淋巴结转移呈正相关。PTEN蛋白的表达与腋窝淋巴结转移呈负相关,而与组织学分级无关(P>0.05)。结论:(1)MMP-9参与乳腺癌的发生发展过程,可作为评估乳腺癌浸润和转移的重要生物学指标。(2)乳腺癌中存在PTEN蛋白表达异常,可能与乳腺癌的发生、发展和预后有关。
关键词(KeyWords)： 乳腺癌;基质金属蛋白酶-9;PTEN蛋白;免疫组织化学
基金项目(Foundation):
作者(Author): 师晓莉;刘存;
Email:
DOI:
参考文献(References)：
- [1]Baker EA,Stephenson TJ,Reed MVR,et al.Expression ofproteinase and inhibitors in human breast cancer progressionand survival[J].Mol Pathol,2002,55:300-304.
- [2]Steck PA,Pershouse MA,Jasser SA,et al.Indentification ofa candicate tumor suppressor gene,MMAC1,at chromosome10q23.3 that is mutated in multiple advanced cancers[J].NatGene,1997,15(5):356-362.
- [3]Lee JO,Yang H,Georgescu MM,et al.Crystal structure ofthe PTEN tumor suppressor:implications for its phosphoinosi-tide phosphatase activity and membrane association[J].Cell,1999,99(3):323-334.
- [4]Tavassoli EF.WHO:Pathology and genetics,Tumours of thebreast and femal genital organs[M].Lyon:LARC Press,2003.10.
- [5]Shimizu M,Saitoh Y,Itoh H.Immunohistochemical stainingof Ha-ras oncogene product in normal,benign,and malignanthuman pancrestic tissue[J].Hum Pathol,1990,21(6):607-612.
- [6]周隽,吴强,杨枫.乳腺癌中PTEN的表达及其临床意义[J].临床与实验病理学杂志,2003,19(2):162-164.
- [7]Liotta LA,Stetler-Stevenson WG.Tumor invasion and metas-tasis:an imbalance of positive and negative regulation[J].Cancer Res,1991,51:5054-5059.
- [8]严志新,邹士林,肖秀娣,等.乳腺癌组织中基质金属蛋白酶-9的表达及其意义[J].江苏大学学报,2003,13(3):205-207.
- [9]Naylor MS,Stamp GM,Pavies BD,et al.Expression and ac-tivity of MMPs and their regulation s in ovarian cancer[J].IntJ Cancer,1995,72:575-582.
- [10]葛凤霞,王海,冯悦年,等.MMP-2,MMP-9和Ⅳ型胶原在乳腺癌中的表达及其意义[J].临床肿瘤学杂志,2003,8(3):164-168.
- [11]张祖平,郭爱萍,王玉龙,等.MMP-9在乳腺癌中的表达及临床意义[J].中国肿瘤临床,2002,29(1):72-73.
- [12]Kame A,Gruis NA,Weaver-Fildhaus J,et al.A cell regula-tor polenlially involved in genesis of many tumor types[J].Sciences,1994,264:436-441.
- [13]Shiozana T,Nikaid T,Shimiza M,et al.Immunohistochemi-cal analysis of the expression of CDK4 and P16 in human en-dometrioia-type endo-metrial cacionoma[J].Cancer,1997,2250-2256.
- [14]Bose S,Crane A,Hibshoosh H,et al.Reduced expression ofPTEN corre-lates with breast cancer progression[J].HumPathol,2002,33(4):405-409.