玻璃化法与程序化法冻融小鼠胚胎复苏及囊胚孵出效果的比较Study on survival rate and hatching rate of mouse embryos after cryopreservation-thawing by vitrification and program freezing
马兰红,蔡霞,胡泊,赵静,巩晓芸
摘要(Abstract)：
目的:探讨玻璃化法与程序化法冻融小鼠胚胎复苏及囊胚孵出效果,为玻璃化法应用于人胚奠定基础。方法:6~8周龄纯净级昆明种小鼠促排卵与雄鼠合笼妊娠后收集2-细胞胚胎,分别采用玻璃化法与程序化法进行冻融及培养,并比较2种方法小鼠胚胎的复苏率、囊胚孵出率。结果:玻璃化法与程序化法的复苏率分别为63.82%(224/351)、62.57%(209/334),差异无统计学意义(P>0.05);玻璃化法与程序化法囊胚孵出率分别为34.2%(120/351)、31.74%(106/334),差异无统计学意义(P>0.05)。结论:玻璃化技术具有有效、简便、迅速的优点,是冻贮鼠胚最为理想的方法。
关键词(KeyWords)： 小鼠;胚胎;玻璃化冷冻;程序冷冻;复苏率;孵出率
基金项目(Foundation):
作者(Author): 马兰红,蔡霞,胡泊,赵静,巩晓芸
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