李琴,娜菲莎·阿里木,席庆,哈晓丹,雷程

• 1:新疆医科大学基础医学院形态中心
• 2:新疆医科大学口腔医学院
• 3:新疆医科大学基础医学院生物教研室
• 4:新疆医科大学第三临床医学院(附属肿瘤医院)

摘要(Abstract)：

目的 探讨结直肠癌组织中细菌的分布及其与miR-23b和自噬相关蛋白ATG12表达的关系。方法 收集新疆医科大学第三临床医学院20例病理诊断明确、临床病历资料完整的结直肠癌患者的癌组织和远端正常组织样本，匀浆后细菌培养，采用16SrDNA扩增子测序的方法检测癌组织与远端正常组织菌群组成，qPCR检测具核梭杆菌(Fn)、miR-23b以及自噬相关蛋白ATG12的表达量。结果 在门水平上，变形菌门、梭杆菌门、厚壁菌门、类杆菌门是结直肠癌患者结直肠组织部位微生物群落中最丰富的菌群，且癌组织中的变形菌门、类杆菌门数量显著高于远端正常组织。在属水平上，副杆菌属、大肠埃氏菌属-志贺氏菌属、链球菌属、维氏菌属、镰刀菌属、拟杆菌属是结直肠癌患者结直肠组织部位微生物群落中最丰富的菌群，且癌组织中的大肠埃氏菌属-志贺氏菌属、镰刀菌属、拟杆菌属数量显著高于远端正常组织，而链球菌、维氏菌属的数量显著低于远端正常组织。qPCR结果显示，结直肠癌组织内Fn mRNA的相对表达量显著高于远端正常组织，差异有统计学意义(P<0.001);miR-23b mRNA(P<0.001)和ATG12 mRNA(P<0.05)的相对表达量显著低于远端正常组织。结论 结直肠癌患者的癌组织和远端正常组织菌群数量的差异与结直肠癌的发生有密切联系，具核梭杆菌是对结直肠癌有较大影响的差异菌属，并且可能与miR-23b、结直肠癌细胞自噬之间存在一定的联系。

关键词(KeyWords)： 结直肠癌;胞内菌;细菌分布;自噬

Abstract:

Keywords:

基金项目(Foundation): 新疆少数民族科技人才特殊培养计划项目(2021D03013)

作者(Author): 李琴,娜菲莎·阿里木,席庆,哈晓丹,雷程

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