一条人类砷相关新cDNA基因的克隆及生物信息学分析Cloning and bioinformatic analysis of a new human arsenic related cDNA gene
吴顺华,杨磊,潘泽民,黄瑾,顾永清,王国荃,郑玉建
摘要(Abstract)：
目的 :探讨砷化物与基因的相互作用 ,克隆砷相关基因 ,利用生物信息学技术分析克隆基因。方法:采用 SMART方法构建 c DNA文库 ,杂交筛选并克隆基因 ,利用生物信息学手段对克隆基因进行结构和功能的分析。6 μm ol/ L Na As O2 处理人正常肝 L- 0 2细胞 2 h,抽提 RNA做基因芯片杂交。结果:成功克隆一条新基因 ,生物信息学分析发现在 7到 4 5 6碱基范围内有一个完整的 ORF,在编码起始区有典型的 Kozak序列 ,编码 14 9个氨基酸的蛋白质。核酸同源性比对发现与人类 1p36 .2 - 36 .3染色体 DNA序列同源性达 98% ,编码蛋白质同 Alu亚家族 SB序列同源性达 85 %。染砷细胞基因芯片杂交发现克隆基因表达增高。结论:克隆的人类砷相关基因 ,编码蛋白质与Alu亚家族 SB序列同源性高。
关键词(KeyWords)： SMART方法;克隆;砷相关基因;生物信息学
基金项目(Foundation): 国家自然科学基金资助项目 ( 3 0 0 60 0 74)
作者(Author): 吴顺华,杨磊,潘泽民,黄瑾,顾永清,王国荃,郑玉建
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