黎飞海,吴军,张文宝,杨梅

• 1:新疆医科大学公共卫生学院劳动卫生与环境卫生教研室
• 2:新疆医科大学基础医学院生物化学与分子生物学教研室
• 3:新疆医科大学第一附属医院临床医学研究院省部共建中亚高发病成因与防治国家重点实验室
• 4:新疆医科大学新疆地方病分子生物学重点实验室

摘要(Abstract)：

目的 探究胆汁酸盐(胆盐)对细粒棘球绦虫(E.granulosus,E.g)发育分化的影响，分析E.gInnexin-unc9 (EgInx)基因在体外有无胆盐-牛磺胆酸钠(Sodium taurocholate)培养的原头蚴(PSC)的表达水平，为研发犬抗E.g疫苗奠定基础。方法 采用含与不含T4009(牛磺胆酸钠的商品型号缩写)的单相培养基分别进行PSC培养，在不同培养时间点对比分析PSC外翻率和发育状态；利用反转录荧光定量PCR(RT-qPCR)和Western Blotting检测不同培养点样本的EgInx相对表达量。结果 不同时间点平行培养的原头蚴外翻情况不同，在培养30 min时和10 d以后，无T4009组的PSC外翻率急剧下降；RT-qPCR结果显示：与从体内刚采集的PSC相比，其EgInx的表达量在培养30 min的T4009组较高，且此时T4009组表达量高于平行无T4009组，且差异具有统计学意义(P<0.05),培养到17 d时，得到类似的结果；Western Blotting和RT-qPCR结果一致。结论 提示在有牛磺胆酸钠的环境中，E.gInnexin-unc9在关键节点表达升高，可能参与调控细粒棘球绦虫的生长发育。

关键词(KeyWords)： 细粒棘球绦虫;牛磺胆酸钠;内联蛋白;表达

Abstract:

Keywords:

基金项目(Foundation): 新疆维吾尔自治区自然科学基金(2017D01C197);; 新疆医科大学博士科研启动金(2019-03)

作者(Author): 黎飞海,吴军,张文宝,杨梅

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