ASICs阻断剂对脑I/R大鼠神经行为学和梗死体积的影响Influence of ASICs blocker on ethology and infarct volume in rats after cerebral focal ischemia and reperfusion
展群岭,张小宁,呼海燕,李华
摘要(Abstract)：
目的:探讨酸敏感离子通道(ASICs)阻断剂阿米洛利(amiloride)对脑缺血/再灌注(ischemia/reperfusion,I/R)大鼠神经行为学和梗死体积的影响。方法:参照ZeaLonga线栓法建立SD大鼠局灶性脑I/R模型。将32只大鼠随机分为4组,即假手术组、I/R模型对照组、amilorideⅠ组和amilorideⅡ组,每组8只,再灌注24h时间点行神经功能缺损评分,之后进行脑梗死灶体积测定和组织细胞HE染色。结果:I/R模型对照组神经功能缺损评分和脑梗死灶体积均明显大于amilorideⅠ组和amilorideⅡ组(P<0.05),amilorideⅠ组明显大于amilo-rideⅡ组(P<0.05)。结论:ASICs阻断剂amiloride对大鼠脑I/R损伤具有保护作用,其作用机制可能主要为amiloride阻断ASICs,抑制Ca2+超载,减轻脑I/R损伤。amiloride药理作用具有剂量效应关系。
关键词(KeyWords)： 缺血再灌注损伤;酸敏感离子通道;阿米洛利
基金项目(Foundation):
作者(Author): 展群岭,张小宁,呼海燕,李华
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