胡夏韵,苏丽萍,路子扬,刘丽,苏天园,蒲红伟

• 1:新疆医科大学基础医学院
• 2:新疆医科大学第一附属医院病理科
• 3:新疆医科大学第三临床医学院
• 4:新疆医科大学公共卫生学院

摘要(Abstract)：

目的探讨二乙酰吗啡致心肌细胞节律改变与心肌钙离子通道异常之间的关系,探究肌钙集蛋白2(CASQ2)与兰碱尼受体2(RYR2)因子与钙通道之间的联系,明确其是否参与心肌细胞钙离子通道异常及心律失常的过程。方法取出生3日龄SD大鼠乳鼠的心肌细胞进行体外培养,分为正常对照组(N组)、二乙酰吗啡干预组(H组)、二乙酰吗啡加维拉帕米干预组(H+V组),二乙酰吗啡浓度为10~(-4) mol/L,药物作用时间24 h。在荧光倒置显微镜下,观察心肌细胞形态和收缩频率节律改变;采用逆转录-聚合酶链反应(RT-PCR)和蛋白印迹法(Western blot)检测CASQ2和RyR2因子的mRNA及蛋白表达,观察H组、N组及H+V组上述2个因子表达含量的变化情况。结果 (1)细胞培养:原代心肌细胞形态多样,呈三角形、长梭形和多边形,细胞核圆形或类圆形,大小正常核膜光滑,细胞间通过伪足相互连接,连接成片的细胞搏动节律一致。(2)心肌细胞形态及节律改变:与N组相比,H组和H+V组心肌细胞形态发生一定程度的改变,收缩频率降低,差异有统计学意义(P<0.05)。(3)与N组相比,H组和H+V组中CASQ2和RYR2蛋白和mRNA表达量减少,差异有统计学意义(P<0.05)。与H组相比,H+V组中CASQ2和RYR2蛋白和mRNA表达量减少,差异有统计学意义(P<0.05)。结论二乙酰吗啡可致心肌细胞自发性搏动频率和节律异常,CASQ2和RyR2因子参与二乙酰吗啡致心肌细胞自发性搏动频率和节律异常的过程。关键字:

关键词(KeyWords)： 二乙酰吗啡;兰碱尼受体2(RYR2);肌集钙蛋白2(CASQ2);心肌细胞;节律异常

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(81860049);; 新疆维吾尔自治区卫生计生委青年医学科技人才专项(WJWY-201827)

作者(Author): 胡夏韵,苏丽萍,路子扬,刘丽,苏天园,蒲红伟

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