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目的比较维吾尔族和汉族散发性乳腺癌患者乳腺癌1号基因(BRCA1)启动子区域甲基化水平及其与临床病理特征的关系。方法采用甲基化特异性PCR法检测维吾尔族和汉族乳腺癌患者BRCA1基因启动子区域甲基化状态,分析甲基化状态改变与乳腺癌发生、发展的关系。结果汉族患者甲基化率为26%,维吾尔族患者甲基化率为30%,汉族与维吾尔族患者甲基化率差异无统计学意义(P=0.65)。BRCA1基因甲基化组与非甲基化组临床病理特征的比较中,汉族患者的年龄及淋巴结状态与BRCA1甲基化有关(P分别为0.021及0.001),维吾尔族患者仅组织学分级与BRCA1甲基化有关(P=0.001)。结论 BRCA1基因表观遗传学改变在肿瘤的发生、发展过程起着重要作用,且与散发性乳腺癌患者的发病年龄及组织学分级和淋巴结状态的发生密切相关。
Abstract：Objective To compare the methylation level of breast cancer gene(BRCA1)promoter region and its relationship to clinical pathological features in the patients with sporadic breast cancer of Uygur and Han patients.Methods The methylation status of BRCA1 gene promoter was detected by methylation specific PCR technique,and the relationship between methylation status and the development of breast cancer was analyzed.Results The methylation rate was 26%in Han patients while it was 30%in Uygur patients,that there was no significant(P =0.65).The age and lymph node status of the patients with BRCA1 methylation and BRCA1 methylation in the BRCA1 gene methylation group were compared with the clinical pathological features(P =0.021 and 0.001).Conclusion Epigenetic changes of BRCA1 gene plays an important role in the development of tumor,and is closely related to the age of onset of the disease and the incidence of lymph node status in patients with sporadic breast cancer.
[1]Xu X,Gammon MD,Zhang Y,et al.BRCA1promoter methylation is associated with increased mortality among women with breast cancer[J].Breast Cancer Res Treat,2009,115(2):397-404.
[2]Pal R,Srivastava N,Chopra R,et al.Investigation of DNA damage response andapoptotic gene methylation pattern in sporadic breast tumors using high throughput quantitative DNA methylation analysis technology[J].Mol Cancer,2010,23(9):303.
[3]Magnani L,Brunelle M,Gévry N,et al.Chromatin landscape and endocrine response in breast cancer[J].Epigenomics,2012,4(6):675-683.
[4]Venkitaraman AR.Cancer suppression by the chromosome custodians,BRCA1and BRCA2[J].Science,2014,343(6178):1470-1475.
[5]Guendel I,Carpio L,Pedati C,et al Methylation of the tumor suppressor protein,BRCA1,influences its transcriptional cofactor function[J].PLoS One,2010,5(6):e11379.
[6]田小侠,司磊.恶性肿瘤发生及发展与DNA甲基化的关系及其检测方法研究进展[J].中国实验诊断学,2005,9(3):474-476.
[7]Tycko B.Epigenetic gene silencing in cancer[J].J Clin Invest,2000,105(4):401-407.
[8]Esteller M.CpG island hypermethylation and tumor suppressor genes:a booming present,a brighter future[J].Oncogene,2002,21(35):5427-5440.
[9]Stefansson OA,Jonasson JG,Olafsdottir K,et al.CpG island hypermethylation of BRCA1and loss of pRb as co-occurring events in basal/triple-negative breast cancer[J].Epigenetics,2011,6(5):638-649.
[10]Jung EJ,Kim IS,Lee EY,et al.Comparison of methylation profiling in cancerous and their corresponding normal tissues from korean patients with breast cancer[J].Ann Lab Med,2013,33(6):431-440.
[11]Holm K,Hegardt C,Staaf J,et al.Meseoarlceh subtypes of breast cancer are associated with characteristic DNA methylation patterns[J].Breast Cancer Res,2010,12(3):R36.
[12]Wu L,Wang F,Xu R,et al.Promoter methylation of BRCA1in the prognosis of breast cancer:a meta-analysis[J].Breast Cancer ResTreat,2013,142(3):619-627.
[13]杨海捷,王丽,魏万里,等.散发性乳腺癌及乳腺不典型导管增生组织BRCA1基因启动子区甲基化分析[J].临床与实验病理学杂志,2008,24(2):141-145.
[14]Karray-Chouayekh S,Trifa F,Khabir A,et al.Clinical significance of epigenetic inactivation of hMLH1and BRCA1in Tunisian patients with invasive breast carcinoma[J].J Biomed Biotechnol,2009;2009:369129.
[15]Jerónimo C,Costa I,Martins MC,et al.Detection of gene promoter hypermethylation in fine needle washings from breast lesions[J].Clin Cancer Res,2003,9(9):3413-3417.
[16]Wei M,Grushko TA,Dignam J,et al.BRCA1promoter hypermethylation in sporadic breast cancer is associated with reduced BRCA1copy number and chromosome 17aneusomy[J].Cancer Res,2005,65(23):10692-10699.
[17]Birgisdottir V,Stefansson OA,Bodvarsdottir SK,et al.Epigenetic silencing and deletion of the BRCA1gene in sporadic breast cancer[J].Breast Cancer Res,2006,8(4):R38.
基本信息:
中图分类号:R737.9
引用信息:
[1]缑文斌,郭媛媛,张巍.维吾尔族和汉族散发性乳腺癌患者BRCA1基因启动子区甲基化的对比研究[J].新疆医科大学学报,2015,38(11):1354-1356+1361.
基金信息:
新疆维吾尔自治区科技厅科技支疆项目(201291172)
2015-11-15
2015-11-15