| 169 | 1 | 2 |
| 下载次数 | 被引频次 | 阅读次数 |
目的探讨正常肝组织与乙型肝炎中TAP1、TAP2、HSP70、HSP90、CNX、β2-MG蛋白表达及相关关系。方法以内源性生物素阻断S-P法来检测32例乙肝患者和16例健康对照者石蜡包埋肝组织的TAP1、TAP2、HSP70、HSP90、CNX、β2-MG蛋白表达情况。结果β2-MG、TAP1、HSP70、HSP90、CNX蛋白在乙肝组表达高于正常组(P均<0.05);通过相关性分析发现,TAP2蛋白与HSP70蛋白在正常组中呈相关性(χ2=16.000,P=0.008)。结论TAP1、TAP2、HSP70、HSP90、CNX、β2-MG蛋白均在乙肝组表达高于正常组(P<0.05),提示这些蛋白均参与MHC-Ⅰ类分子的乙肝病毒抗原处理及提呈过程,并且可以做为诊断乙肝的临床指标。
Abstract：Objective To evaluate the expression levels of the MHC class Ⅰantigen presentation-associated proteins: transport associated antigen-processing proteins TAP1 and TAP2,heat shock protein(HSP)70 and HSP90,calnexin(CNX),and β2 microglobulin(β2-MG),in normal and hepatitis B liver tissues,and its association with hepatitis B.Methods The expression of proteins TAP1,TAP2,HSP70,HSP90,CNX,and β2-MG in paraffin-embedded liver tissues from 32 patients with hepatitis B(as hepatitis B group) and 16 healthy individuals(as control group) were detected by streptoavidin-biotin-peroxidase(S-P) based immunohistochemistry method with blocking endogenous biotin.Results The expression level of β2-MG,TAP1,HSP70,HSP90 and CNX in liver tissues from hepatitis patients was higher than that in normal group(P<0.05).The correlation analysis suggested a correlative relationship between expression of TAP2 and HSP70 in the normal liver tissues(χ2=16.000,P=0.008).Conclusion The expression of TAP1,TAP2,HSP70,HSP90,CNX,and β2-MG proteins was higher in the hepatitis B group than that in the normal group(P<0.05).This results suggest that these proteins are involved in the process and presentation of MHC class Ⅰ antigens of hepatitis B virus,and could be used as markers for clinical diagnosis of hepatitis B.
[1]韦颖华,吴继周.乙肝疫苗免疫应答与主要组织相容性抗原MHC表达关系的研究进展[J].内科杂志,2007,2(1):62-65.
[2]李坤,戴悦,阴秀丽等.中国华北地区人群TAP1基因多态性与乙肝关联性的研究[J].胃肠病学和肝病学杂志,2005,14(1):66-70.
[3]Elefsiniotis IS,Moulakakis A,Pantazis KD,et al.Relation-ship between serum b2-microglobulin levels and virologicalbreakthroughin HBeAg-negative chronic hepatitis B patients,under long-termtreat ment schedules including lamivudine[J].World J Gastroenterol,2005,11(13):1922-1928.
[4]Yegane S,Revanli M,Taneli F,et al.The role of beta2 mi-croglobulin levels in monitoring chronic hepatitis B[J].To-hoku J Exp Med,2004,203(1):53-57.
[5]Matsui M,Machida S,Itani-Yohda T,et al.Downregulationof the proteasome subunits,transporter,and antigen presenta-tionin hepatocellular carcinoma,and their restoration byinter-feron-gamma[J].Gastroenterol Hepatol,2002,8(17):897-907.
[6]王萃?,王晓花,孙成刚.抗原提呈相关转运体2基因多态性与乙型肝炎病毒感染的关系—附110例检测分析[J].新医学,2007,38(8):507-508.
[7]夏天,郝妍,王小华,等.抗原肽运载体TAP2基因与乙型肝炎病毒感染预后的相关性研究[J].中华微生物学与免疫学杂志,2005,25(4):331-337.
[8]Xu C,Qi S,Gao L,et al.Genetic polymorphisms of LMP/TAP gene and hepatitis B virus infection risk in the Chinesepopulation[J].J Clin I mmunol,2007,27(5):534-541.
[9]Forsythe HL,Jarvis JL,Turner J W,et al.Stable associationof HSP90 and p23,but not hsp70,with active human telomer-ase[J].J Biol Chem,2001,276:15571-15574.
[10]蒋业贵,王宇明.慢性乙型肝炎肝组织中主要热休克蛋白的表达及意义[J].中华肝脏病学杂志,2003,11(6):365.
基本信息:
中图分类号:R512.62
引用信息:
[1]海力曼.衣拉洪,海来提.居来提,伊力木努尔.甫拉提,等.MHC-Ⅰ类分子抗原呈递相关蛋白与乙肝相关性研究[J].新疆医科大学学报,2010,33(02):124-127.
基金信息:
国家自然科学基金资助课题(30460046)
2010-02-28
2010-02-28