| 121 | 6 | 34 |
| 下载次数 | 被引频次 | 阅读次数 |
目的研究分析喀什木纳格葡萄树伤流液中多糖的分离纯化及其单糖组成成分。方法采用醇沉法提取多糖,Sevage法脱蛋白,经DEAE-52纤维素及SephedexG-150凝胶柱分离纯化,采用气相色谱分析法测定单糖组成。结果 80%乙醇沉淀、Sevage法除蛋白得粗多糖;葡萄树伤流液粗多糖经过DEAE-52分离纯化分别得到GBSK-1、GBSK-2、GBSK-3、GBSK-4、GBSK-5、GBSK-6、GBSK-7和GBSK-8共8个多糖组分。其中4个多糖组分经过SephadexG-150纯化后,其洗脱峰均为单峰;通过GC分析得出GBSK-3的单糖组成较简单,主要由木糖组成;多糖GBSK-2、GBSK-5的单糖组成相似,主要由鼠李糖、阿拉伯糖、甘露糖、葡萄糖、半乳糖组成;多糖GBSK-1的单糖组成主要由鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖、半乳糖组成。结论该方法较好地分离纯化葡萄树伤流液多糖。GC分析适用于多糖中的单糖组成分析,为葡萄树伤流液的开发和利用提供了科学依据。
Abstract：Objective To separate and purify of Kaxigar munaka grapevine bleeding sap polysaccharides and analysis of monosaccharide composition.Methods Prepare crude polysaccharide using alcohol precipitation and by improved Sevage method.Purify the obtained crude polysaccharide by DEAE-52 and Sephadex G-150 column chromatography.Analysis the monosaccharide composition by gas chromatography.Results To get crude polysaccharide by 80% alcohol precipitation and to remove proteins by Sevage method.The crude polysaccharide was separated by DEAE-52,and obtained 8 polysaccharides,respectively GBSK-1,GBSK-2,GBSK-3,GBSK-4,GBSK-5,GBSK-6,GBSK-7and GBSK-8.After being purified,4polysaccharides eluting peaks were unimodal.From the result obtained by GC analysis,monosaccharide composition of GBSK-3 was relatively simple and mainly composed of xylose.The polysaccharides GBSK-2,GBSK-5 had similar monosaccharide composition and they were mainly composed of rhamnose,arabinose,mannose,glucose and galactose.While the polysaccharides GBSK-1was mainly composed of rhamnose,arabinose,xylose,mannose,glucose and galactose.Conclusion Results indicate that this method is suitable for the separation and purification of grapevine bleeding sap polysaccharide.It is suitable to analysis the monosaccharide composition of grapevine bleeding sap polysaccharides by gas chromatography.A scientific basis is provided for the development and utilization of grapevine bleeding sap.
[1]徐德源,王健,任水莲,等.新疆葡萄产品的优势及其生态候条件评价[J].新疆气象,2004,27(1):16-19.
[2]伊力哈木·托合迪,买吐孙·买提肉孜,艾力·阿巴白克力.沙枣树胶恢复变质葡萄树伤流液抗氧化能力的生物活性作用研究[J].现代农业科学,2009,16(3):32-34.
[3]艾尔肯·依不拉音,阿吉姑·阿布都热西提,李建光,等.新疆葡萄树伤流液抗氧化活性及热稳定性的研究[J].食品与发酵工业,2009,35(1):14-16.
[4]王传现,钟进义.葡萄多酚对活性氧自由基的清除作用研究[J].营养学报,2001,23(2):170-173.
[5]朱富荣,伊力哈木·托合提,艾尔肯·依不拉音,等.新疆葡萄树伤流液抗氧化活性及多酚含量测定[J].食品研究与开发,2013,34(9):12-15.
[6]布热米古丽·买买提,艾尔肯·依不拉音,朱富荣,等.新疆葡萄树伤流液中总糖含量的比较研究[J].西北药学杂志,2013,28(3),224-226.
[7]艾尔肯·依不拉音,朱富荣,布热米古丽·买买提,等.新疆葡萄树伤流液中矿质元素含量的主成分及聚内分析[J].食品与发酵工业,2013,39(6):208-211.
[8]Schepetkin IA,Quin MT.Botanical polysaccharides:macrophage immuneomo-dulation and therapeutic potential[J].Int Immunopharmacol,2006,6(3):317-333.
[9]Tao YW,Tian GY.Studies on the physicochemical Properties,Structure and antitumor activity of polysaccharide YhPS-1from the root of Cordalis yanhusuo Wang[J].Chin J Chem,2006,24(2):235-239.
[10]宗根,陈鸿珊,郭志敏等.牛膝多糖硫酸酯体外和体内抗艾滋病病毒作用[J].药学学报,2008,43(7):702-706.
[11]Ge Y,Duan YF,Fang GZ,et al.Study on biological activities of Physalis alkekengi var francheti polysaccharide[J].J Sci Food Agric,2009,89(9):1593-1598.
[12]段国睁,欧阳臻,樊一桥,等.茅苍术多糖防治小鼠高血糖的实验研究[J].中华中医药学刊,2008,26(6):1211-1212.
[13]曹蕊,曹玉华.米糠多糖的提取及其抗UVB辐射研究[J].天然产物研究与开发,2008,20(3):518-521.
[14]依巴代提·托乎提,玛依努尔·吐尔逊,苏巴提·吐尔地,等.阿里红多糖对氧自由基的清除作用[J].新疆医科大学学报,2006,29(1):15-17.
[15]潘九英,陈慧瑾,许颖.中药多糖药理作用研究的新进展[J].中华实用中西医杂志,2007,20(19):1744-1745.
[16]Wang ZP,Gulledge J,Zheng JQ,et al.Physical injury stimulates aerobic methane emissionsfrom trrestrial plants[J].Biogeosciences,2009,6(4):615-621.
[17]于小蓉,孙莲,孟磊,等.新疆药桑叶多糖中单糖组成的气相色谱分析[J].西北药学杂志,2013,28(1):25-27.
基本信息:
中图分类号:R284.1
引用信息:
[1]艾尔肯·依不拉音,布热米古丽·买买提,黄琼.喀什木纳格葡萄树伤流液多糖的分离纯化及其单糖组成分析[J].新疆医科大学学报,2013,36(11):1558-1561.
基金信息:
国家自然科学基金(21162030)
2013-11-15
2013-11-15