新疆医科大学学报

目的探讨急、慢性期布鲁菌病(简称布病)患者miRNA-155、miRNA-21及Th1、Th2、Th17相关细胞因子的表达差异,了解布病的免疫特点。方法收集急、慢性布病患者73例以及健康对照者30例血标本,采用CBA法测定其Th1类细胞因子(IFN-γ、TNF-α、IL-18)、Th2类细胞因子(IL-10、IL-33)以及Th17类细胞因子(IL-17)表达水平,同时对其中30例急、慢性布病患者以及15例健康对照者采用荧光实时定量PCR(qRT-PCR)法检测其血标本miRNA-155和miRNA-21的表达情况。采集上述研究对象实验室检查(ESR、CRP)结果,对以上各结果进行统计学分析。结果急、慢性组Th1类细胞因子(IFN-γ、TNF-α、IL-18)、Th2类细胞因子(IL-10、IL-33)和Th17细胞因子(IL-17)表达水平均升高(P均<0.05);其中,Th1和Th17类细胞因子在急性期升高显著,Th2类细胞因子在慢性期增高显著(P均<0.05);急、慢性组miRNA-155和miRNA-21表达均上升(P均<0.05);miRNA-155在急性组升高显著;IL-18的表达与IFN-γ、TNF-α表达呈正相关,IL-33的表达与IL-10表达呈正相关;miRNA-155表达与Th1、Th17类细胞因子以及CRP、ESR均呈正相关。结论患者感染布鲁菌后,机体免疫应答在急、慢性期存在差异,急性期以miRNA-155、Th1和Th17类细胞因子表达为主,慢性期以Th2类细胞因子表达为主;miRNA155与Th1、Th17类细胞因子表达以及布病炎症水平(CRP、ESR)密切关联,推测miRNAs可能影响Th细胞的分化,进而参与布病的发生和发展。

Objective To explore the expression differences of miRNA-155, miRNA-21,Th1, Th2 and Th17 related cytokines in patients with acute and chronic brucellosis, and to understand the immune characteristics of brucellosis.Methods Blood samples were collected from 73 patients with acute and chronic brucellosis and 30 healthy controls.The expression levels of Th1 cytokines( IFN-γ, TNF-α, IL-18), Th2 cytokines( IL-10, IL-33) and Th17 cytokines(IL-17) were detected by CBA. The expression of miRNA-155 and miRNA-21 of 30 patients with acute and chronic brucellosis and 15 healthy controls were detected by qRT-PCR. The results of ESR and CRP of all above subjects were collected, and followed by detection data was analysed and processed by statistical software. Results The expression levels of Th1 cytokines( IFN-γ, TNF-α, IL-18), Th2 cytokines( IL-10, IL-33) and Th17 cytokines( IL-17) in acute and chronic groups were both increased(P<0.05); Th1 and Th17 increased significantly in acute phase while Th2 increased significantly in chronic phase(P<0.05); The expression of miRNA-155 and miRNA-21 increased in acute and chronic groups(P<0.05). miRNA-155 increased significantly in acute group; IL-18 was positively correlated with IFN-γ and TNF-α, while IL-33 was positively correlated with IL-10; miRNA-155 was positively correlated with Th1,Th17 cytokines, CRP and ESR. Conclusion After infection with Brucella, immune response of the patients are different in acute and chronic phase. The expression of miRNA-155, Th1 and Th17 cytokines are mainly in acute phase, and Th2 cytokines is mainly in chronic phase. The expression of miRNA155 is closely related to Th1, Th17 cytokines and brucellosis inflammation levels( CRP, ESR). It speculates miRNAs may affect the differentiation of Th cells, and then participates in occurrence and development of brucellosis.