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目的探讨长链非编码RNA ITGB1(lncRNA ITGB1)在乳腺癌组织中的表达及其临床意义。方法选择2017年1月-2018年12月新疆医科大学附属肿瘤医院乳腺外科经病理确诊的90例乳腺癌患者。所有患者术前未接受放化疗、内分泌治疗及靶向治疗,术后病理确诊为乳腺癌原发性浸润性癌,收集其组织及配对癌旁组织(取自肿瘤组织>5 cm的乳腺正常组织)。采用qRT-PCR法检测lncRNA ITGB1的表达水平,并分析其与临床病理资料的相关性。结果 lncRNA ITGB1在乳腺癌组织中的表达水平(8.25±3.12)明显高于癌旁组织(1.31±0.25)(P=0.000)。lncRNA ITGB1的表达与TNM分期(P=0.000)和腋窝淋巴结转移(P=0.000)明显相关,但与年龄、肿瘤大小、雌激素受体(ER)、孕激素受体(PR)以及分化程度无关。结论 lncRNA ITGB1可能在乳腺癌起癌基因的作用,高表达的lncRNA ITGB1可作为乳腺癌诊断的一个潜在的新型生物标志物。
Abstract：Objective To investigate the expression and clinical significance of long-chain non-coding RNA ITGB1 in breast cancer tissues. Methods 90 patients with breast cancer diagnosed by pathology in the Affiliated Tumor Hospital of Xinjiang Medical University were selected. All patients received no radiotherapy and chemotherapy, endocrine therapy and targeted therapy before surgery. The postoperative pathology was diagnosed as primary invasive breast cancer. 90 patients with breast cancer tissue and matched paracancerous tissues were collected(from tumor tissue >5 cm). The expression of lncRNA ITGB1 in breast cancer and matched paracancerous tissues has been detected by qRT-PCR, and its correlation with clinicopathological data of breast cancer was analyzed. Results The expression level of ITGB1 in lnc-ITGB breast cancer tissues(8.25±3.12) was higher than that in adjacent tissues(1.31±0.25), and the difference was statistically significant(t=18.551, P<0.001). The expression of lncRNA ITGB1 was correlated with TNM stage(P<0.001) and axillary lymph node metastasis(P<0.001). However, there were no correlation between the expression level of lncRNA ITGB1 and age, tumor size, estrogen receptor(ER), progesterone receptor(PR) and degree of differentiation(P>0.05). Conclusion lncRNA ITGB1 may play a role in tumor suppressor gene in breast cancer, and high expression of lncRNA ITGB1 may be a potential novel biomarker for breast cancer diagnosis.
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基本信息:
中图分类号:R737.9
引用信息:
[1]赵倩,杨亮,朱丽萍.长链非编码RNA ITGB1在乳腺癌组织中的表达及其临床意义[J].新疆医科大学学报,2019,42(07):865-867+872.
基金信息:
新疆维吾尔自治区自然科学基金(2017D01C379)
2019-03-07
2019
2019-04-25
2019
1
2019-06-26
2019-06-26