新疆医科大学学报

目的 联合运用超高效液相色谱-四极杆-飞行时间质谱联用(Ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry, UPLC-Q-TOF-MS)与气相色谱-质谱联用(Gas chromatography-mass spectrometry, GC-MS)技术鉴定骆驼峰复合油膏所含中药材和骆驼峰油中的化学成分，并研究骆驼峰复合油膏、骆驼峰复合油膏所含中药材混合提取物和骆驼峰油的体外抗氧化活性。方法 采用UPLC-Q-TOF-MS定性分析骆驼峰复合油膏所含中药材混合提取物的化学成分；采用GC-MS分析骆驼峰油的脂肪酸组成；通过1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl, DPPH)自由基、2,2′-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)[2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid),ABTS]阳离子自由基及羟自由基清除实验，综合评价骆驼峰复合油膏、骆驼峰油、骆驼峰复合油膏所含中药材混合提取物体外抗氧化能力。结果 骆驼峰复合油膏所含中药材混合提取物组分中共鉴定出77种化合物，包括黄酮类、酚酸类、生物碱类及姜辣素类等多种活性成分；从骆驼峰油中鉴定出33种游离脂肪酸，其中顺式-9-十八碳烯酸(30.41%)、棕榈酸(30.13%)和硬脂酸(18.13%)为主要成分。体外抗氧化结果显示，与骆驼峰复合油膏所含中药材混合提取物、骆驼峰油相比，骆驼峰复合油膏的DPPH自由基、ABTS阳离子自由基和羟自由基清除能力更强，其半数抑制浓度值分别为102.9μg/mL、224.0μg/mL及294.9μg/mL。结论 本研究从抗氧化角度初步揭示了骆驼峰油与中药材提取物配伍的合理性，为其临床应用与深度开发提供了实验依据

Objective Ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS) and gas chromatography-mass spectrometry(GC-MS) were employed to identify the Chinese medicinal materials contained in camel hump composite oil ointment and the chemical components in camel hump oil, and to investigate the in vitro antioxidant activities of camel hump composite oilointment, mixed extracts of Chinese medicinal materials contained in camel hump composite oilointment, and camel hump oil. Methods UPLC-Q-TOF-MS was used to qualitatively analyze the chemical components of the mixed herbal extract contained in camel hump compound oil ointment. The fatty acid composition of camel hump oil was analyzed by GC-MS. The in vitro antioxidant capacities of the camel hump compound ointment, camel hump oil, and the mixed extract of Chinese medicinal ingredients contained in Camel Hump Compound Ointment were comprehensively evaluated through 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS) cation radical, and hydroxyl radical scavenging assays. Results A total of 77 compounds were identified in the mixed extract components of traditional Chinese medicines contained in camel hump compound ointment, including various active ingredients such as flavonoids, phenolic acids, alkaloids, and capsaicinoids. 33 free fatty acids were identified in camel hump oil, with cis-9-octadecenoic acid(30.41%), palmitic acid(30.13%), and stearic acid(18.13%) being the main components. In vitro antioxidant results showed that compared with the mixed extract of Chinese medicinal materials and camel hump oil contained in camel hump compound oil ointment, camel hump compound oil ointment demonstrated stronger DPPH radical, ABTS cation radical, and hydroxyl radical scavenging capacity, with IC₅₀ values of 102.9 μg/mL, 224.0 μg/mL, and 294.9 μg/mL, respectively. Conclusion This study preliminarily reveals the rationality of the compatibility between camel hump oil and traditional Chinese medicine extracts from an antioxidant perspective, providing an experimental basis for its clinical application and in-depth development.