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目的:探索不同照射模式对人鼻咽低分化鳞癌细胞株(CNE-2)放射敏感性的影响。方法:运用克隆形成实验(courtenay assay),检测不同照射模式下CNE-2细胞的存活分数(SF),拟合细胞存活曲线。并运用四氮唑蓝法(MTT)进一步验证克隆形成实验的结果。结果:分别给予CNE-2细胞0、1、2、4、6、8 Gy的照射后,CNE-2细胞的SF值随照射剂量增加逐渐下降,并且在相同的剂量点,模拟调强照射(IMRT)组的SF均高于常规照射组;根据线性二次方程拟合CNE-2细胞存活曲线,α常规>αIMRT,β常规>βIMRT,N常规 Objective:To explore the influence of different radiation pattern on radiosensitivity in cell strain(CNE-2) of nasopharyngeal squamous cell carcinoma.Methods:Surviving fraction(SF) was analyzed by courtenay assay,fitting cell survival curve;Cell growth inhibition was measured by MTT.Results:There were some different radiation doses including 0,1,2,4,6,8 Gy,SF was decreasing with the radiation dose rised.Compared with simulation IMRT(SIMRT) group,SF of convention radiation(CR) group was lower in every dose point;Fitting cell survival curve by linear quadratic equation and multi-target one-shot model,αCR>αSIMRT,βCR>βSIMRT,NCR
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基本信息:
中图分类号:R739.6
引用信息:
[1]黄莉,王若峥,李品东.不同照射模式对人鼻咽低分化鳞癌细胞株CNE-2放射敏感性的影响研究[J].新疆医科大学学报,2008,31(11):1493-1497.
基金信息:
国家自然科学基金资助项目(3066046);; 新疆维吾尔自治区自然基金资助(200721115);; 新疆医科大学创新基金(2006-20)
2008-11-30
2008-11-30